Biochemistry
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L'échelle du laboratoire: Production et purification d'un anticorps thérapeutique
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Ce protocole décrit la production d'un anticorps thérapeutique dans un système d'expression mammalien. Les procédés décrits comprennent la préparation d'ADN vecteur, la transfection stable et l'adaptation d'une lignée de cellules embryonnaires de rein 293 humaine sans sérum, mis en place des cultures à grande échelle et la purification par chromatographie d'affinité.
Tags
Biochimie numéro 119 Biosimilaire des anticorps des anticorps thérapeutiques la production d'anticorps la production de la protéine des cellules HEK-293 la transfection la purification des protéines la Chromatographie d'affinité la FPLCRelated Videos
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