Journal
/
Bioengineering
/
Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier

Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier

DOI:
10.3791/55181-v

•

10:16 min

•

February 08, 2017

•

, , , ,
1Center for Biomolecular Science and Engineering,Naval Research Laboratory, 2Optical Sciences Division,Naval Research Laboratory, 3Sotera Defense Solutions

Chapters

  • 00:05Title
  • 00:51Conjugation of PEG-Chol to DiO-LCNP via EDC Coupling and Characterization of the Product
  • 02:33Preparation of Cell Culture Dishes for Delivery Experiments and Imaging
  • 04:07Cellular Delivery of DiO and DiO-LCNPs and Imaging of Fixed Cells
  • 06:11Cellular Delivery DiO-LCNPs and DiI FRET Imaging in Live Cells
  • 07:37Results: Analysis of DiO-LCNP-PEG-Chol Cellular Uptake to the Plasma Membrane and Quantification of Cytotoxicity
  • 08:57Conclusion

Summary

Automatic Translation

Automatic Translation

A liquid crystal nanoparticle (LCNP) nanocarrier is exploited as a vehicle for the controlled delivery of a hydrophobic cargo to the plasma membrane of living cells.

Tags

Keywords: Liquid Crystal NanoparticleHydrophobic CargoPlasma Membrane DeliveryPEG Cholesterol ConjugationGel ElectrophoresisHEK 293 T17 Cell Culture

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/55181?article_type=v

Contrast Ultrasound Targeted Treatment of Gliomas in Mice via Drug-Bearing Nanoparticle Delivery and Microvascular Ablation
check_url/55181?article_type=v

Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation
check_url/55181?article_type=v

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
check_url/55181?article_type=v

Hydrogel Nanoparticle Harvesting of Plasma or Urine for Detecting Low Abundance Proteins
check_url/55181?article_type=v

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging
check_url/55181?article_type=v

Alternating Magnetic Field-Responsive Hybrid Gelatin Microgels for Controlled Drug Release
check_url/55181?article_type=v

Preparation of Monodomain Liquid Crystal Elastomers and Liquid Crystal Elastomer Nanocomposites
check_url/55181?article_type=v

Synthesis of Biocompatible Liquid Crystal Elastomer Foams as Cell Scaffolds for 3D Spatial Cell Cultures
check_url/55181?article_type=v

Real-time Visualization and Analysis of Chondrocyte Injury Due to Mechanical Loading in Fully Intact Murine Cartilage Explants
check_url/55181?article_type=v

In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth

Read Article