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MALDI 이미징 질량 분석에 대한 쥐 뇌 조직에 강글리오사이드의 감지 및 시각화를위한 DAN 매트릭스의 승화
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Sublimation of DAN Matrix for the Detection and Visualization of Gangliosides in Rat Brain Tissue for MALDI Imaging Mass Spectrometry
DOI:

08:36 min

March 23, 2017

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Chapters

  • 00:05Title
  • 00:42Tissue Preparation and Sectioning
  • 02:08Sublimator Apparatus Set-up
  • 04:57Sublimation
  • 06:57Results: Matrix-assisted Laser Desorption/Ionization (MALDI) Imaging Mass Spectrometry (IMS) of Gangliosides in the Rat Brain
  • 07:31Conclusion

Summary

Automatic Translation

A protocol for the sublimation of DAN matrix onto rat brain tissue for the detection of gangliosides using MALDI Imaging Mass Spectrometry is presented.

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