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JoVE Journal
Neuroscience

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Imaging Neurons within Thick Brain Sections Using the Golgi-Cox Method
 

Imaging Neurons within Thick Brain Sections Using the Golgi-Cox Method

Article DOI: 10.3791/55358-v 10:26 min April 18th, 2017
April 18th, 2017

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Summary

We present a protocol for using the Golgi-Cox staining method in thick brain sections, in order to visualize neurons with long dendritic trees contained within single tissue samples. Two variants of this protocol are also presented that involve cresyl violet counterstaining, and the freezing of unprocessed brains for long-term storage.

Tags

Keywords: Golgi-Cox Method Neuron Morphology Brain Sectioning Cryoprotection Vibratome Sectioning Histology Neuroscience Rodent Brain
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