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厚脑切片使用高尔基考克斯方法中成像神经元
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Imaging Neurons within Thick Brain Sections Using the Golgi-Cox Method
DOI:

10:26 min

April 18, 2017

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Chapters

  • 00:05Title
  • 00:39Golgi-Cox Impregnation of Brains
  • 01:47Brain Sectioning
  • 03:34Developing Brain Sections
  • 05:03Mounting Brain Sections
  • 06:55Imaging Stained Neurons within Thick Brain Sections
  • 08:21Results: Photomicrographs of Stained Neurons in the Anterior Cingluate Area 1
  • 09:44Conclusion

Summary

Automatic Translation

我们提出了一个协议,用于使用所述高尔基体考克斯染色法在厚的脑切片中,为了可视化与包含单个组织样品中的树突状长树木神经元。此协议的两种变型还提出涉及甲酚紫复染,和未加工的大脑进行长期存储的冻结。

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