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Neuroscience
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厚脑切片使用高尔基考克斯方法中成像神经元
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Imaging Neurons within Thick Brain Sections Using the Golgi-Cox Method
Please note that all translations are automatically generated.
Click here for the English version.
厚脑切片使用高尔基考克斯方法中成像神经元
DOI:
10.3791/55358-v
•
10:26 min
•
April 18, 2017
•
Emma L. Louth
,
Charles D. Sutton
,
Ari L. Mendell
,
Neil J. MacLusky
,
Craig D.C. Bailey
1
Department of Biomedical Sciences, Ontario Veterinary College
,
University of Guelph
Chapters
00:05
Title
00:39
Golgi-Cox Impregnation of Brains
01:47
Brain Sectioning
03:34
Developing Brain Sections
05:03
Mounting Brain Sections
06:55
Imaging Stained Neurons within Thick Brain Sections
08:21
Results: Photomicrographs of Stained Neurons in the Anterior Cingluate Area 1
09:44
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们提出了一个协议,用于使用所述高尔基体考克斯染色法在厚的脑切片中,为了可视化与包含单个组织样品中的树突状长树木神经元。此协议的两种变型还提出涉及甲酚紫复染,和未加工的大脑进行长期存储的冻结。
Tags
Keywords: Golgi-Cox Method
Neuron Morphology
Brain Sectioning
Cryoprotection
Vibratome Sectioning
Histology
Neuroscience
Rodent Brain
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