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的转录分析<em>ç</em>。<em>线虫</em> RNA测序数据通过炫套房的银河项目

JoVE Journal
Genetics

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JoVE Journal Genetics

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

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的转录分析<em>ç</em>。<em>线虫</em> RNA测序数据通过炫套房的银河项目

DOI:

10.3791/55473-v

•

10:19 min

•

April 08, 2017

•

Francis R. G. Amrit, Arjumand Ghazi

¹Department of Pediatrics,University of Pittsburgh School of Medicine, Children’s Hospital of Pittsburgh

Chapters

00:05Title
00:56Getting Started with Galaxy
03:28Data Analysis with Tuxedo Suite
06:19Gene Ontology (GO) Term Analyses Using DAVID
07:56Results: Transcriptome Dictated by the Transcription Elongation Factor TCER-1, that Extends Lifespan of Germline-less C. elegans
09:39Conclusion

Summary

Automatic Translation

银河和大卫已经成为流行的工具，让没有生物信息学培训调查分析和解释RNA-Seq的数据。我们描述了一种协议，用于秀丽隐杆线虫的研究人员进行RNA测序实验，访问和使用银河处理数据集，并获得从使用DAVID的基因列表有意义的生物信息。

Tags

C. Elegans RNA Sequencing Transcriptomic Analysis Tuxedo Suite Galaxy Project Bioinformatics Differential Gene Expression Gene Ontology NGS RNA Analysis FASTQ Data Upload Workflow

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