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一个定制HPLC纯化协议，产量高纯度淀粉样蛋白β42和贝塔淀粉样蛋白肽40，有能力寡聚物形成的

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

A Tailored HPLC Purification Protocol That Yields High-purity Amyloid Beta 42 and Amyloid Beta 40 Peptides, Capable of Oligomer Formation

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一个定制HPLC纯化协议，产量高纯度淀粉样蛋白β42和贝塔淀粉样蛋白肽40，有能力寡聚物形成的

DOI:

10.3791/55482-v

•

06:34 min

•

March 27, 2017

•

Christopher J. A. Warner, Subrata Dutta, Alejandro R. Foley, Jevgenij A. Raskatov

¹Department of Chemistry,University of California, Santa Cruz

Chapters

00:05Title
00:34Preparative HPLC Purification of the Aβ40 or Aβ42 Peptide
02:17Purification of Aβ Peptide
03:26Analytical HPLC Analysis of the Purified Aβ Protein
04:42Results: HPLC Measurements of Purified Aβ42 Peptide
05:53Conclusion

Summary

Automatic Translation

在此，我们报告说，得到高纯度的β淀粉样蛋白42（Aβ42）和β淀粉样蛋白40（Aβ40）肽，能够形成低聚物量身定制的HPLC纯化协议。 β淀粉样蛋白在阿尔茨海默氏症牵连高度易聚集，疏水肽。该肽的淀粉样蛋白性质使得其净化是一个挑战。

Tags

Keywords: HPLC Purification Amyloid Beta 42 Amyloid Beta 40 Oligomer Formation Alzheimer’s Disease High Purity Peptides Hydrophobic Stationary Phase PLRP-S High PH Conditions

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