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En skräddarsydd HPLC reningsprotokoll som ger hög renhet Amyloid Beta 42 och Amyloid Beta 40 Peptider som kan oligomerbildning
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
A Tailored HPLC Purification Protocol That Yields High-purity Amyloid Beta 42 and Amyloid Beta 40 Peptides, Capable of Oligomer Formation
DOI:

06:34 min

March 27, 2017

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Chapters

  • 00:05Title
  • 00:34Preparative HPLC Purification of the Aβ40 or Aβ42 Peptide
  • 02:17Purification of Aβ Peptide
  • 03:26Analytical HPLC Analysis of the Purified Aβ Protein
  • 04:42Results: HPLC Measurements of Purified Aβ42 Peptide
  • 05:53Conclusion

Summary

Automatic Translation

Häri rapporterar vi en skräddarsydd HPLC reningsprotokoll som ger hög renhet amyloid beta 42 (Aβ42) och amyloid beta 40 (Aβ40) peptider som kan oligomerbildning. Amyloid beta är en i hög grad aggregation benägen, hydrofob peptid inblandad i Alzheimers sjukdom. Den amyloidogeniska karaktären av peptiden gör dess rening en utmaning.

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