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Technieken voor het induceren en te kwantificeren Cellulaire senescentie
JoVE Journal
Biology
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JoVE Journal Biology
Techniques to Induce and Quantify Cellular Senescence
DOI:

06:51 min

May 01, 2017

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Chapters

  • 00:05Title
  • 00:43DNA Damage-induced Senescence
  • 01:40Cell Staining for Senescence-associated (SA) Beta-Galactosidase
  • 03:30Quantifying SA Secretory Phenotype (SASP) Protein Levels
  • 04:37Results: Representative SA-beta-Galactosidase Staining and SASP Expression Analyses
  • 05:42Conclusion

Summary

Automatic Translation

Cellular veroudering, de onomkeerbare toestand van de celcyclus, kan worden geïnduceerd door verschillende cellulaire stress. Hier beschrijven we protocollen bij cellulaire veroudering en methoden ertoe te brengen markers van veroudering te beoordelen.

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