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变凹形形状 Microwells 的配对磁珠和磁体阵列
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Bioengineering
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JoVE Journal Bioengineering
A Paired Bead and Magnet Array for Molding Microwells with Variable Concave Geometries
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变凹形形状 Microwells 的配对磁珠和磁体阵列

DOI:
10.3791/55548-v

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11:42 min

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January 28, 2018

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, ,
1School of Mechanical Engineering, College of Engineering,Chung-Ang University

Chapters

  • 00:00Title
  • 01:55Fabricating the Aluminum Plates
  • 02:40Aligning the Magnets
  • 03:29Preparing the Platform
  • 05:50PDMS Microwell Fabrication
  • 08:29Cell Culture
  • 10:17Results
  • 11:29Conclusion

Summary

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本文介绍了一种在不需要复杂的高成本设备的情况下, 制作凹 microwells 的鲁棒方法。采用磁力、钢珠和通孔阵列, 在 3 cm x 3 cm 烷 (microwells) 衬底上形成几百个。

Tags

Keywords: 3D Cell CultureConcave MicrowellsMagnetic Bead ArrayMicrowell FabricationSpherical Microstructures

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