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通过使用多光谱成像流式细胞术测量LC3，p62和LAMP1共定位评估自噬通量
JoVE Journal
Biology
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JoVE Journal
Biology
Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
Please note that all translations are automatically generated.
Click here for the English version.
通过使用多光谱成像流式细胞术测量LC3，p62和LAMP1共定位评估自噬通量
DOI:
10.3791/55637-v
•
11:39 min
•
July 21, 2017
•
Haley R. Pugsley
1
Amnis Biology Department
,
MilliporeSigma
Chapters
00:05
Title
00:42
Jurkat Cell LC3, LAMP1, and p62 Labeling
02:18
Multispectral Imaging Flow Cytometry (MIFC)
03:43
MIFC Analysis
08:35
Results: Representative MIFC Analyses
10:28
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里，具有分析特征的多光谱成像流式细胞术可以比较3个自噬标记的明亮细节图像，并量化其共定位以及LC3斑点计数，用于以客观，定量和统计学鲁棒的方式测量自噬。
Tags
Autophagy
LC3
P62
LAMP1
Colocalization
Multispectral Imaging Flow Cytometry
Quantification
Jurkat Cells
Formalin
Permeabilization
DAPI
Laser Power
Dot Plot
Cell Population
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