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Rewiring Neuronal Circuits: A New Method for Fast Neurite Extension and Functional Neuronal Connection
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Neuroscience
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JoVE Journal Neuroscience
Rewiring Neuronal Circuits: A New Method for Fast Neurite Extension and Functional Neuronal Connection

Rewiring Neuronal Circuits: A New Method for Fast Neurite Extension and Functional Neuronal Connection

DOI:
10.3791/55697-v

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10:26 min

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June 13, 2017

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, , , , ,
1Department of Physics,McGill University, 2Department of Neurology and Neurosurgery,Montreal Neurological Institute, 3Ananda Devices

Chapters

  • 00:05Title
  • 01:01Standardization of Neuronal Cultures Using Microfluidic Devices: Device Assambly
  • 02:54Plating Neurons in Microfluidic Systems
  • 04:47PDL-bead Adhesion and Micromanipulation
  • 06:13Pulling Neurites and Connecting Neurons
  • 08:45Results: The Newly Induced, Elongated, and Connected Neurite Can Transfer Information Between Two Isolated Neuronal Populations
  • 09:39Conclusion

Summary

Automatic Translation

Automatic Translation

This procedure describes how to rapidly initiate, extend and connect neurites organized in microfluidic chambers using poly-D-lysine-coated beads fixed to micropipettes that guide neurite elongation.

Tags

Keywords: Neurite ExtensionFunctional Neuronal ConnectionNeurite Growth RateSynapse FormationNeural NetworkSingle-cell ManipulationExperimental ReproducibilityPDL CoatingMicrofluidic DevicesCell CultureNeuroscience

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