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Neuroscience
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重建神经元电路:快速神经延伸和功能性神经元连接的新方法
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Rewiring Neuronal Circuits: A New Method for Fast Neurite Extension and Functional Neuronal Connection
Please note that all translations are automatically generated.
Click here for the English version.
重建神经元电路:快速神经延伸和功能性神经元连接的新方法
DOI:
10.3791/55697-v
•
10:26 min
•
June 13, 2017
•
Margaret H. Magdesian
2,3
,
Madeleine Anthonisen
,
G. Monserratt Lopez-Ayon
,
Xue Ying Chua
,
Matthew Rigby
,
Peter Grütter
1
Department of Physics
,
McGill University
,
2
Department of Neurology and Neurosurgery
,
Montreal Neurological Institute
,
3
Ananda Devices
Chapters
00:05
Title
01:01
Standardization of Neuronal Cultures Using Microfluidic Devices: Device Assambly
02:54
Plating Neurons in Microfluidic Systems
04:47
PDL-bead Adhesion and Micromanipulation
06:13
Pulling Neurites and Connecting Neurons
08:45
Results: The Newly Induced, Elongated, and Connected Neurite Can Transfer Information Between Two Isolated Neuronal Populations
09:39
Conclusion
Summary
Automatic Translation
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Automatic Translation
该程序描述了如何使用固定在引导神经突伸长的微量移液管上的聚-D-赖氨酸包被的珠快速启动,延伸和连接在微流体室中组织的神经突。
Tags
Keywords: Neurite Extension
Functional Neuronal Connection
Neurite Growth Rate
Synapse Formation
Neural Network
Single-cell Manipulation
Experimental Reproducibility
PDL Coating
Microfluidic Devices
Cell Culture
Neuroscience
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