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监测ER / SR钙释放与目标Ca<sup> 2+</sup>传感器捕捉器<sup> +</sup
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Monitoring ER/SR Calcium Release with the Targeted Ca2+ Sensor CatchER+
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监测ER / SR钙释放与目标Ca<sup> 2+</sup>传感器捕捉器<sup> +</sup

DOI:
10.3791/55822-v

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12:30 min

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May 19, 2017

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, , ,
1Department of Chemistry, Center of Diagnostics and Therapeutics (CDT),Georgia State University

Chapters

  • 00:05Title
  • 01:30Transfection of HEK293 Cells
  • 02:43Transfection of C2C12 Myoblast Cells
  • 04:10Preparation of Slide and Fluorescence Microscope
  • 06:54Imaging Drug-induced Ca2+ Transients and In Situ Kd Calibration
  • 10:14Results: In Situ Kd of CatchER+ in C2C12 Myoblast Cells
  • 11:32Conclusion

Summary

Automatic Translation

Automatic Translation

我们提出了使用我们的靶向基因编码钙指示剂（GECI）CatchER +的方案 ，用于使用实时荧光显微镜监测HEK293和骨骼肌C2C12细胞的内质网/肌浆网（ER / SR）中的快速钙瞬变。还讨论了原位测量和校准的协议。

Tags

ER/SR Calcium ReleaseCatchER+HEK293 CellsC2C12 CellsCalcium SignalingCalcium TransientsGECICell TransfectionFluorescence ImagingCalcium-related Diseases

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