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Tre-dimensionelle Tissue Engineering justeret Astrocyte netværk for at sammenfatte udviklingsmæssige mekanismer og lette nervesystem regenerering
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1Center for Brain Injury & Repair, Department of Neurosurgery, Perelman School of Medicine,University of Pennsylvania, 2Center for Neurotrauma, Neurodegeneration & Restoration,Michael J. Crescenz Veterans Affairs Medical Center, 3School of Biomedical Engineering,Drexel University, 4Department of Bioengineering, School of Engineering and Applied Sciences,University of Pennsylvania, 5Neuroscience Graduate Group, Perelman School of Medicine,University of Pennsylvania
Chapters
- 00:05Title
- 01:03Agarose Hydrogel Micro-Column Fabrication
- 03:51ECM Core Fabrication
- 05:26Seeding Micro-columns with Astrocytes
- 06:32Results: Formation of Astrocyte Bundles within Hydrogel Micro-columns over Time
- 07:54Conclusion
Vi fremvise udviklingen af selvsamlede, tre-dimensionelle bundter langs justeret astrocytic somata og processer inden for en roman biomateriale encasement. Disse manipuleret "levende stilladser", udstiller micron-skala diameter, endnu udvide centimeter i længden, kan tjene som prøvestande til at studere udviklingsforstyrrelser mekanismer eller lette neuroregeneration ved at lede neuronal migration og/eller cytoskeletale pathfinding.
Tags
Astrocyte NetworksAligned AstrocytesNervous System RegenerationMicrogel Micro-columnsGlial TubesRostral Migratory StreamAgarose HydrogelIn Vitro Neuro-biological ModelNeuro-regenerative TherapyCell MigrationAxon PathfindingGlial-mediated DevelopmentTraumaNeurodegenerative DiseaseCentral Nervous SystemRegenerationTreatment StrategiesNeuroanatomyGlia-mediated MechanismsAcupuncture NeedleCapillary TubeMicro-column BoatsAgarose SolutionDPBSMicro Scalpel
