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1Department of Medicine, Institute of Translational Pharmacology,Italian National Research Council, 2Epigenetics and Regenerative Medicine,IRCCS Fondazione Santa Lucia, 3Biological and Environmental Sciences and Engineering Division,King Abdullah University of Science and Technology (KAUST), 4Department of Life Sciences,University of Modena and Reggio Emilia
Chapters
- 00:05Title
- 01:00Muscle Injury and Tissue Harvest
- 03:12Cryosectioning, Immunostaining, and Confocal Microscopy Imaging
- 06:47Results: Representative Injured Muscle Tissue and Muscle Satellite Cell (MuSC) Analyses
- 07:43Conclusion
Активная аутофагия связана с продуктивной регенерацией мышц, которая необходима для активации мышечной стволовой клетки (MuSC). Здесь мы предоставляем протокол для обнаружения in situ LC3, маркера аутофагии в MyoD-положительных MuSCs участков мышечной ткани у контрольных и раненых мышей.
