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Establishing Dual Resistance to EGFR-TKI and MET-TKI in Lung Adenocarcinoma Cells In Vitro with a 2-step Dose-escalation Procedure
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JoVE Journal Cancer Research
Establishing Dual Resistance to EGFR-TKI and MET-TKI in Lung Adenocarcinoma Cells In Vitro with a 2-step Dose-escalation Procedure

Establishing Dual Resistance to EGFR-TKI and MET-TKI in Lung Adenocarcinoma Cells In Vitro with a 2-step Dose-escalation Procedure

DOI:
10.3791/55967-v

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09:38 min

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August 11, 2017

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, , ,
1Institute of Molecular Oncology,Showa University, 2Center for Biotechnology,Showa University

Chapters

  • 00:05Title
  • 00:30Determining Initial Gefitnib Concentration Using the 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenylterazolium Bromide (MTT) Assay
  • 02:15Continuous Stepwise Dose-escalation of Gefitnib Treatment of PC-9 Cells
  • 03:31Determine Initial Concentration of PHA665752 Using the MTT Assay
  • 06:37Results: Two-step Dose-escalation Procedure to Establish Dual Resistance to EGFT-TKI and MET-TKI
  • 08:21Conclusion

Summary

Automatic Translation

Automatic Translation

An in vitro method for establishing dual resistance to an EGFR-TKI and a MET-TKI in cancer cells is described. This method is useful for developing treatments for patients with EGFR-mutations, who exhibit disease progression despite EGFR-TKI treatment with MET-amplification. It can also be modified for inhibitors targeting other molecules.

Tags

Lung AdenocarcinomaEGFR-TKI ResistanceMET-TKI Resistance2-step Dose-escalationDrug Resistance MechanismCell CultureMTT AssayGefitinibPHA-665752PC-9 CellsMET1000 Cells

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