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Bestemmelse af celleoverfladeekspression og endocytisk proteinhastighed i primære astrocytkulturer ved anvendelse af biotinylering
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation
DOI:

10:42 min

July 03, 2017

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Chapters

  • 00:05Title
  • 00:51Determining Relative Cell-surface Protein Expression in Astrocytes by Biotinylation
  • 04:40Determining Internalization Rate of Cell-surface Proteins in Astrocytes by Biotinylation
  • 08:52Results: AQP4 Expression at the Cell-surface in the Presence of Laminin and AQP4 Internalization in Astrocytes
  • 10:15Conclusion

Summary

Automatic Translation

To biotinyleringsbaserede metoder, der er designet til bestemmelse af celleoverfladeekspression og endocytisk hastighed af proteiner udtrykt i plasmamembranen, er præsenteret i denne rapport.

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