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小鼠第二腭融合的实时成像
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Developmental Biology
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JoVE Journal Developmental Biology
Live Imaging of Mouse Secondary Palate Fusion
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小鼠第二腭融合的实时成像

DOI:
10.3791/56041-v

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06:10 min

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July 27, 2017

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, ,
1Department of Cell and Tissue Biology, Program in Craniofacial Biology and Institute for Human Genetics,University of California San Francisco, 2Laboratory of Transgenic Models Diseases,Czech Centre for Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences

Chapters

  • 00:05Title
  • 00:42Palate Explant Culture and Confocal Time-lapse Imaging
  • 03:28Results: Representative Live Palate Explant Culture Imaging
  • 05:16Conclusion

Summary

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在这里，我们提出了使用共聚焦显微镜的小鼠二次腭融合的活体成像方案。该方案可以与各种荧光报告小鼠系列结合使用，并可与途径抑制剂一起用于机械性的洞察。该协议可适用于其他开发系统中的实时成像。

Tags

Live ImagingMouseSecondary Palate FusionConfocal MicroscopyCranial Facial DevelopmentPalatal ShelvesMidline Epithelia SeamEpithelial ConvergenceExplant Culture

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