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Developmental Biology
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小鼠第二腭融合的实时成像
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Live Imaging of Mouse Secondary Palate Fusion
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小鼠第二腭融合的实时成像
DOI:
10.3791/56041-v
•
06:10 min
•
July 27, 2017
•
Seungil Kim
,
Jan Prochazka
,
Jeffrey O. Bush
1
Department of Cell and Tissue Biology, Program in Craniofacial Biology and Institute for Human Genetics
,
University of California San Francisco
,
2
Laboratory of Transgenic Models Diseases
,
Czech Centre for Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences
Chapters
00:05
Title
00:42
Palate Explant Culture and Confocal Time-lapse Imaging
03:28
Results: Representative Live Palate Explant Culture Imaging
05:16
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出了使用共聚焦显微镜的小鼠二次腭融合的活体成像方案。该方案可以与各种荧光报告小鼠系列结合使用,并可与途径抑制剂一起用于机械性的洞察。该协议可适用于其他开发系统中的实时成像。
Tags
Live Imaging
Mouse
Secondary Palate Fusion
Confocal Microscopy
Cranial Facial Development
Palatal Shelves
Midline Epithelia Seam
Epithelial Convergence
Explant Culture
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