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JoVE Journal Neuroscience
Assessment of Dopaminergic Homeostasis in Mice by Use of High-performance Liquid Chromatography Analysis and Synaptosomal Dopamine Uptake
 

Assessment of Dopaminergic Homeostasis in Mice by Use of High-performance Liquid Chromatography Analysis and Synaptosomal Dopamine Uptake

Article DOI: 10.3791/56093-v • 11:26 min • September 21st, 2017
September 21st, 2017 •
1, 1, 2, 1, 1
1Molecular Neuropharmacology and Genetics Laboratory, Lundbeck Foundation Center for Biomembranes in Nanomedicine, Department of Neuroscience and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, 2Laboratory of Neuropsychiatry, Psychiatric Center Copenhagen and Department of Neuroscience and Pharmacology, University of Copenhagen

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Summary
Automatic Translation

Synaptosomal dopamine uptake and high-performance liquid chromatography analysis represent experimental tools to investigate dopamine homeostasis in mice by assessing the function of the dopamine transporter and levels of dopamine in striatal tissue, respectively. Here we present protocols to measure dopamine tissue content and assess the functionality of the dopamine transporter.

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Keywords: Dopamine Dopamine Transporter HPLC Synaptosomal Dopamine Uptake Dopamine Homeostasis In Vivo Manipulation Brain Dissection Tissue Homogenization Synaptosome Preparation Dopamine Uptake Assay
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