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JoVE Journal
Bioengineering

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Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays
 

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Article DOI: 10.3791/56230-v 10:44 min November 13th, 2017
November 13th, 2017

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Summary

We demonstrate a snap chip technology for performing cross-reactivity-free multiplexed sandwich immunoassays by simply snapping two slides. A snap apparatus is used for reliably transferring reagents from microarray-to-microarray. The snap chip can be used for any biochemical reactions requiring colocalization of different reagents without cross-contamination.

Tags

Keywords: Sandwich Immunoassays Multiplexed Assays Cross-reactivity Spotter-free Protein Biomarkers Microarray Alignment Marks Nitrocellulose Functionalized Glass Capture Antibody Detection Antibody Polysorbate 20 BSA Blocking Storage Double-transfer Method
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