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Assessing Urinary Tract Junction Obstruction Defects by Methylene Blue Dye Injection
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Assessing Urinary Tract Junction Obstruction Defects by Methylene Blue Dye Injection

Assessing Urinary Tract Junction Obstruction Defects by Methylene Blue Dye Injection

DOI:
10.3791/56247-v

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06:05 min

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October 12, 2017

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1Cancer and Developmental Biology Laboratory, National Cancer Institute,National Institutes of Health

Chapters

  • 00:05Title
  • 00:47Methylene Blue Dye Preparation
  • 01:26Dissection of Prenatal Embryos
  • 03:13Dye Injection and Flow Monitoring
  • 04:50Results: Methylene Blue Dye Identifies Urinary System Deficiencies
  • 05:36Conclusion

Summary

Automatic Translation

Automatic Translation

Methylene blue dye injection into the renal pelvis facilitates the assessment of urinary tract junction obstruction defects during mouse embryonic urinary tract development. Here, a protocol for methylene blue dye injection into the renal pelvis is described.

Tags

Keywords: Urinary Tract Junction ObstructionMethylene Blue Dye InjectionKidney DevelopmentUretal Bladder ConnectionPerinatal EmbryosAbdominal Cavity DissectionGenotypingDye Injection

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