/
/
Produktion af gensplejsede gyldne syriske hamstere af Pronuclear injektion af CRISPR/Cas9-komplekset
A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Animal, Dairy, and Veterinary Sciences,Utah State University, 2National Centre for International Research in Cell and Gene Therapy, Sino-British Research Centre, School of Basic Medical Sciences,Zhengzhou University, 3Department of Animal Science Division of Applied Life Science (BK21 Plus),Gyeongsang National University, 4Institute of Agriculture and Life Science,Gyeongsang National University, 5Centre for Molecular Oncology, Barts Cancer Institute,Queen Mary University of London
Chapters
- 00:05Title
- 01:06Zygote Isolation
- 03:31PN Injection
- 04:52Results: Evaluation of Gene Targeting in Golden Syrian Hamster by the Cas9/sgRNA System
- 05:36Conclusion
Pronuclear (PN) injektion af den grupperede regelmæssigt interspaced korte palindromiske gentagelser (CRISPR) og CRISPR-forbundet protein-9 nukleasen (CRISPR/Cas9) system er en yderst effektiv metode til fremstilling af gensplejsede gyldne syriske hamstere. Heri, beskriver vi detaljeret PN injektion protokollen til produktion af genet knockout hamstere med CRISPR/Cas9-systemet.
