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Üretim CRISPR/Cas9 kompleks Pronuclear enjeksiyonla genetiği altın Suriye hamster
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1Department of Animal, Dairy, and Veterinary Sciences,Utah State University, 2National Centre for International Research in Cell and Gene Therapy, Sino-British Research Centre, School of Basic Medical Sciences,Zhengzhou University, 3Department of Animal Science Division of Applied Life Science (BK21 Plus),Gyeongsang National University, 4Institute of Agriculture and Life Science,Gyeongsang National University, 5Centre for Molecular Oncology, Barts Cancer Institute,Queen Mary University of London
Chapters
- 00:05Title
- 01:06Zygote Isolation
- 03:31PN Injection
- 04:52Results: Evaluation of Gene Targeting in Golden Syrian Hamster by the Cas9/sgRNA System
- 05:36Conclusion
Pronuclear (PN) iğne kümelenmiş düzenli olarak kısa palindromik yineler (CRISPR) interspaced ve CRISPR ilişkili protein-9 nükleaz (CRISPR/Cas9) sistemi genetiği altın Suriye hamster üretmek için son derece verimli bir yöntemdir. Burada, biz gene nakavt hamster CRISPR/Cas9 sistemi ile üretim için detaylı PN enjeksiyon Protokolü tanımlamak.
