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Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)
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Chapters
- 00:05Title
- 00:56Growth and Enumeration of Bacteria
- 02:38Preparation of Highly Dilute Samples for DHM and Recording DHM Videos
- 04:06Calculations of Cell Density and Limits of Detection
- 05:22Results: Growth Curves and Representative DHM Data
- 06:14Conclusion
Digital holographic microscopy (DHM) is a volumetric technique that allows imaging samples 50-100X thicker than brightfield microscopy at comparable resolution, with focusing performed post-processing. Here DHM is used for identifying, counting, and tracking microorganisms at very low densities and compared with optical density measurements, plate count, and direct count.
Tags
Keywords:
Digital Holographic MicroscopyMicroorganismsLow ConcentrationsBacterial DetectionVolumetric TechniqueThree-dimensional ImagingPathogen DetectionBlood Stream InfectionCerebrospinal Fluid InfectionPetroff-Hausser Counting ChamberLive And Dead Cell EnumerationSerial DilutionColony-forming UnitsLysogeny Broth Media
