A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Clinical Research Division,Fred Hutchinson Cancer Research Center, 2Hematology Branch, National Heart, Lung and Blood Institute,National Institutes of Health, 3Epigenetics Program, Department of Cell and Developmental Biology,University of Pennsylvania Perelman School of Medicine, 4Departments of Medicine and Biochemistry,University of Washington
Chapters
- 00:04Title
- 01:17Selecting Single Cell Clones and Characterizing MeCP2 Reporter Expression
- 04:31Screening the Library for Reactivation Using Hygromycin B Selection
- 06:44Validating the Identified Hairpins ( Hits )
- 09:27Results: Isolation of Clones with the Reporter on the Inactive X Chromosome and Characterization of Hygromycin Resistance
- 10:17Conclusion
Wir berichten über eine kleine Hairpin RNA (ShRNA) und die nächste Generation Sequencing-basiertes Protokoll zur Identifizierung von Regulatoren der X-Chromosom Inaktivierung in einem murinen Zelllinie mit Firefly Luciferase und Hygromycin-Resistenz-Gene verschmolzen, das Methyl CpG verbindliches Protein 2) MeCP2) gen auf dem x-Chromosom inaktiv.
