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Chapters
- 00:08Introduction
- 00:27CellChip preparation: Hydrophilization, coating, incubation
- 03:41Bioreactor Setup
- 07:43Incubation Setup
- 08:58Analysis of organotypic functions
Descriviamo un chip basato su piattaforma per il tridimensionale coltura di cellule in micro-bioreattori. Un chip in grado di ospitare fino a 10 milioni. cellule che possono essere coltivate in condizioni ben definite in relazione al flusso del fluido, tensione ecc ossigeno in una sterile, la circolazione ad anello chiuso.
Tags
Chip-basedThree-dimensional Cell CulturePerfused Micro-bioreactorsChipNon-biodegradable PolymersBio-degradable PolymersDimensionsCubic Micro-containersRound RecessesScaffoldCellsNutrient SupplyGas SupplyBioreactor HousingClosed Steril Circulation LoopRoller PumpMedium ReservoirPerfusionSuperfusionMixed Operation ModeCell LinesPrimary CellsOrganotypic FunctionsHepatocellular Carcinoma Cell LinesLiver Specific Genes
