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RANKL 小鼠骨髓破骨细胞培养法研究 mTORC1 在破骨细胞形成中的作用
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation
DOI:

09:37 min

March 15, 2018

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Chapters

  • 00:05Title
  • 00:59Dissection and Isolation
  • 03:27Plating and Differentiation
  • 05:41Tartrate Resistant Acid Phosphatase Staining and Activity Quantification of Culture Medium
  • 07:25Results: Deletion of Raptor Impaired Osteoclast Formation
  • 08:27Conclusion

Summary

Automatic Translation

本手稿描述了一种从小鼠骨髓中分离和培养破骨细胞的体外的协议, 并研究雷帕霉素复合物1在破骨细胞形成中的作用。

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