/

/

透射电镜下体的样品制备与成像

JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology

Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy

Please note that all translations are automatically generated. Click here for the English version.

透射电镜下体的样品制备与成像

DOI:

10.3791/56482-v

•

11:15 min

•

January 04, 2018

•

Min Kyo Jung, Ji Young Mun

¹Department of Convergence Medicine, University of Ulsan College of Medicine and Asan Institute for Life Sciences,Asan Medical Center, ²Synaptic Circuit Plasticity Laboratory, Department of Structure and Function of Neural Network,Korea Brain Research Institute

Chapters

00:05Title
01:14Exosome Block Preparation
03:38Sectioning, Staining, and Imaging of the Exosome
04:27Immuno-staining of Sections and Imaging
07:24Whole Mount for Immunostaining
09:10Results: Immunostained Exosome Sections Provide Insight to Their Protein Makeup
09:59Conclusion

Summary

Automatic Translation

本协议描述了传输电子显微镜所需的各种技术, 包括阴性染色, 超薄切片的详细结构, 和免疫金标签, 以确定特定的蛋白质在体的位置。

Tags

Exosomes Transmission Electron Microscopy Sample Preparation Imaging Extracellular Vesicles Protein Localization Ultracentrifugation Fixation Dehydration Embedding Low Viscosity Embedding Disease Diagnosis Microcellular Incubation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

电子显微镜对胚胎的制备果蝇胚胎心管

单病毒原子力显微镜和超分辨率荧光成像的样品制备

低温电子显微镜样品制备借助于聚焦离子束

在造血干细胞和祖细胞的成年小鼠颅骨骨髓体内四维跟踪

串联高压冷冻和植物组织中快速冷冻替代透射电子显微镜

该做什么和不该做什么低温电子显微镜的：入门样品制备和高质量的数据采集为大分子三维重建

准备厚生物样品的观察扫描透射电子断层扫描

透射电镜的小型化样品制备方法

高压冷冻、微波辅助对比度增强和最小树脂嵌入在体积成像中的生物样品制备

使用新型机器人系统 Spotiton 进行时间解决研究的低温电子微观网格准备

Read Article