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应用实时 PCR 技术检测血清和血浆拷贝数

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Cancer Research

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JoVE Journal Cancer Research

Serum and Plasma Copy Number Detection Using Real-time PCR

Please note that all translations are automatically generated. Click here for the English version.

应用实时 PCR 技术检测血清和血浆拷贝数

DOI:

10.3791/56502-v

•

09:21 min

•

December 15, 2017

•

Samanta Salvi, Vincenza Conteduca, Filippo Martignano, Giorgia Gurioli, Daniele Calistri, Valentina Casadio

¹Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS

Chapters

00:05Title
00:55Collection of Serum or Plasma
02:20Isolating Cell-free (cfDNA) from Serum or Plasma
05:15Real-time PCR and Analysis of cfDNA
07:36Results: Detection of Androgen Receptor (AR) Copy Number Variations in cfDNA of Metastatic Castration-resistant Prostate Cancer (CRPC) Patients
08:25Conclusion

Summary

Automatic Translation

这篇手稿描述的拷贝数变异分析进行血清或血浆 DNA 使用 real-time PCR 方法。该方法适用于抗去势前列腺癌患者的耐药性预测, 但也可为其他疾病提供信息。

Tags

Serum Plasma Copy Number Detection Real-time PCR Cell-free DNA Androgen Receptor Gene Copy Number Variation Metastatic Castration-resistant Prostate Cancer Biomarker Liquid Biopsy Digital PCR

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