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用于内耳组织工程的 Decellularizing 小鼠耳蜗协议
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Bioengineering
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JoVE Journal Bioengineering
A Protocol for Decellularizing Mouse Cochleae for Inner Ear Tissue Engineering
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用于内耳组织工程的 Decellularizing 小鼠耳蜗协议

DOI:
10.3791/56523-v

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09:53 min

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January 01, 2018

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, , , ,
1Department of Otolaryngology,University of Kansas Medical Center, 2Stephenson School of Biomedical Engineering,University of Oklahoma, 3Department of Plastic Surgery,University of Kansas Medical Center

Chapters

  • 00:05Title
  • 01:05Temporal Bone Harvest and Cochlea Isolation
  • 03:07Cochlear Processing
  • 05:11Infusion of hWJCs into Decellularized Cochleae
  • 06:58Results: Cell Culture on Decellularized, Decalcified Mouse Cochleae
  • 08:18Conclusion

Summary

Automatic Translation

Automatic Translation

本协议的目的是演示一种有效的方法, decellularize 和 decalcify 小鼠耳蜗作为组织工程应用的支架使用。

Tags

Keywords: DecellularizationDecalcificationMouse CochleaExtracellular MatrixStem Cell DifferentiationSensory EpitheliumTemporal BoneBony LabyrinthStapesOval WindowRound WindowPerilymph1% SDS SolutionPBSAntibiotic-antimycoticPenicillin-streptomycin

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