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Bioengineering
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内耳組織工学用マウス蝸牛を Decellularizing するためのプロトコル
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Bioengineering
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JoVE Journal
Bioengineering
A Protocol for Decellularizing Mouse Cochleae for Inner Ear Tissue Engineering
Please note that all translations are automatically generated.
Click here for the English version.
内耳組織工学用マウス蝸牛を Decellularizing するためのプロトコル
DOI:
10.3791/56523-v
•
09:53 min
•
January 01, 2018
•
Christopher A. Neal
,
Jennifer G. Nelson-Brantley
,
Michael S. Detamore
,
Hinrich Staecker
,
Adam J. Mellott
1
Department of Otolaryngology
,
University of Kansas Medical Center
,
2
Stephenson School of Biomedical Engineering
,
University of Oklahoma
,
3
Department of Plastic Surgery
,
University of Kansas Medical Center
Chapters
00:05
Title
01:05
Temporal Bone Harvest and Cochlea Isolation
03:07
Cochlear Processing
05:11
Infusion of hWJCs into Decellularized Cochleae
06:58
Results: Cell Culture on Decellularized, Decalcified Mouse Cochleae
08:18
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコルの目的は、decellularize し、めしマウス蝸牛組織工学用の足場として利用する効果的な方法を示すことです。
Tags
Decellularization
Decalcification
Mouse Cochlea
Extracellular Matrix
Stem Cell Differentiation
Sensory Epithelium
Temporal Bone
Bony Labyrinth
Stapes
Oval Window
Round Window
Perilymph
1% SDS Solution
PBS
Antibiotic-antimycotic
Penicillin-streptomycin
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