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Pre-Conditioning the Airways of Mice with Bleomycin Increases the Efficiency of Orthotopic Lung Cancer Cell Engraftment
JoVE Journal
Medicine
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JoVE Journal Medicine
Pre-Conditioning the Airways of Mice with Bleomycin Increases the Efficiency of Orthotopic Lung Cancer Cell Engraftment
DOI:

09:02 min

June 28, 2018

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Chapters

  • 00:04Title
  • 00:48Bleomycin and Cell Bolus Treatment
  • 03:31Engrafting Lung Adenocarcinoma Cell Lines
  • 05:04Image Analysis to Monitor Tumor Growth
  • 06:33Results: Analysis of Bleomycin Pre-treatment in Athymic Mice
  • 07:58Conclusion

Summary

Automatic Translation

We describe a method to significantly enhance orthotopic engraftment of lung cancer cells into the murine lungs by pre-conditioning the airways with injury. This approach may also be applied to study stromal interactions within the lung microenvironment, metastatic dissemination, lung cancer co-morbidities, and to more efficiently generate patient derived xenografts.

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