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固定化的线虫线虫分析神经元内细胞的转运

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Developmental Biology

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JoVE Journal Developmental Biology

Immobilization of Caenorhabditis elegans to Analyze Intracellular Transport in Neurons

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固定化的线虫线虫分析神经元内细胞的转运

DOI:

10.3791/56690-v

•

07:35 min

•

October 18, 2017

•

Shinsuke Niwa

¹Frontier Research Institute for Interdisciplinary Sciences and Graduate School of Life Sciences,Tohoku University

Chapters

00:05Title
00:34Experimental Preparation
02:00Mounting of Worms
02:56Imaging
05:23Results: Visualization of Axonal and Intraflagellar Transport in Neurons
06:53Conclusion

Summary

Automatic Translation

线虫线虫(线虫) 是研究轴突和细胞内转运的良好模型。在这里, 我描述了一个协议的在体内记录和分析轴突和 intraflagellar 运输在C. 线虫。

Tags

Keywords: Caenorhabditis Elegans Intracellular Transport Neurons Axonal Transport Intraflagellar Transport Kinesin Dynein Microtubules Green Fluorescent Protein In Vivo Live Imaging Agarose Gel

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