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Chapters
- 00:04Title
- 00:46Production of AAV in HEK 293 Cells by Plasmid DNA Transfection
- 02:50Recovery of Viral Particles, and Treatment with Serratia marcescens Endonuclease and Proteinase K
- 04:20Phenol-choloroform Extraction, Ethanol Precipitation, and Preparation of Plasmid DNA Standards
- 05:55Dot Blot Assay
- 08:46Hybridization and Washing
- 10:50Results: AAV Titration and Functional Assessment of AAP Proteins Using a Quantitative Dot Blot Assay
- 12:58Conclusion
Dette manuskript detaljer en ligetil dot skamplet assay til kvantitering af adeno-associeret virus (AAV) titers og dens anvendelse til at studere rolle aktivering af forsamling proteiner (AAPs), en roman klasse af ikke-strukturelle virale proteiner, der findes i alle AAV serotyper, fremme samling af capsids stammer fra beslægtet og heterolog AAV serotyper.
