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Chapters
- 00:04Title
- 00:46Production of AAV in HEK 293 Cells by Plasmid DNA Transfection
- 02:50Recovery of Viral Particles, and Treatment with Serratia marcescens Endonuclease and Proteinase K
- 04:20Phenol-choloroform Extraction, Ethanol Precipitation, and Preparation of Plasmid DNA Standards
- 05:55Dot Blot Assay
- 08:46Hybridization and Washing
- 10:50Results: AAV Titration and Functional Assessment of AAP Proteins Using a Quantitative Dot Blot Assay
- 12:58Conclusion
