JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Cancer Research
/
病人衍生软组织肉瘤原代培养的建立
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Cancer Research
Establishment of a Primary Culture of Patient-derived Soft Tissue Sarcoma
Please note that all translations are automatically generated.
Click here for the English version.
病人衍生软组织肉瘤原代培养的建立
DOI:
10.3791/56767-v
•
07:55 min
•
April 11, 2018
•
Alessandro De Vita
,
Laura Mercatali
,
Giacomo Miserocchi
,
Chiara Liverani
,
Chiara Spadazzi
,
Federica Recine
,
Alberto Bongiovanni
,
Federica Pieri
,
Davide Cavaliere
,
Valentina Fausti
,
Dino Amadori
,
Toni Ibrahim
1
Osteoncology and Rare Tumors Center
,
Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS
,
2
Pathology Unit
,
Morgagni-Pierantoni Hospital
,
3
Unit of Surgery and Advanced Oncologic Therapies
,
Morgagni-Pierantoni Hospital
Chapters
00:04
Title
01:23
Tumor Specimen Collection and Processing
04:03
Cytological Sample Preparation
04:41
STS Cell Culture
05:42
Results: Patient-derived Primary Culture of Liposarcoma Cells
06:41
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
以下协议的重点是建立一个初级文化的病人衍生软组织肉瘤 (STS)。该模型可以帮助我们更好地了解这些罕见的恶性肿瘤的分子背景和药理学特征, 并可作为进一步研究的出发点, 以改善 STS 管理。
Tags
Primary Culture
Patient-derived
Preclinical Model
Pathophysiology
Therapy
Drug Testing
Cell Isolation
Tumor Heterogeneity
Collagenase Digestion
TRIzol Preservation
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
可追踪记者乳腺癌患者来源的移植瘤贴标肿瘤生长和转移的研究
来自沙柯玛患者衍生异种移植物的肿瘤启动细胞的分离和表征
黑色素瘤患者衍生异种移植模型
建立胃癌患者衍生异种移植模型和原细胞系
患者衍生胰腺腺腺腺腺癌有机体模型的分离和特征
斑马鱼原发性患者衍生肿瘤异种移植物的药物筛选
患者衍生的中枢神经系统转移异种移植模型的建立与利用
建立胰腺癌斑马鱼患者来源的异种移植物进行化学敏感性测试
间变性甲状腺癌和头颈部鳞状细胞癌患者来源异种移植模型的建立与表征
患者来源乳腺类器官的建立和培养
Read Article