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病人衍生软组织肉瘤原代培养的建立
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Cancer Research
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JoVE Journal Cancer Research
Establishment of a Primary Culture of Patient-derived Soft Tissue Sarcoma
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病人衍生软组织肉瘤原代培养的建立

DOI:
10.3791/56767-v

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07:55 min

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April 11, 2018

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, , , , , , , , , , ,
1Osteoncology and Rare Tumors Center,Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, 2Pathology Unit,Morgagni-Pierantoni Hospital, 3Unit of Surgery and Advanced Oncologic Therapies,Morgagni-Pierantoni Hospital

Chapters

  • 00:04Title
  • 01:23Tumor Specimen Collection and Processing
  • 04:03Cytological Sample Preparation
  • 04:41STS Cell Culture
  • 05:42Results: Patient-derived Primary Culture of Liposarcoma Cells
  • 06:41Conclusion

Summary

Automatic Translation

Automatic Translation

以下协议的重点是建立一个初级文化的病人衍生软组织肉瘤 (STS)。该模型可以帮助我们更好地了解这些罕见的恶性肿瘤的分子背景和药理学特征, 并可作为进一步研究的出发点, 以改善 STS 管理。

Tags

Primary CulturePatient-derivedPreclinical ModelPathophysiologyTherapyDrug TestingCell IsolationTumor HeterogeneityCollagenase DigestionTRIzol Preservation

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