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检测暴露于有机氯农药的肝细胞线粒体功能的实验方案

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Experimental Protocol for Detecting Mitochondrial Function in Hepatocytes Exposed to Organochlorine Pesticides

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检测暴露于有机氯农药的肝细胞线粒体功能的实验方案

DOI:

10.3791/56800-v

•

08:39 min

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September 16, 2020

•

Qian Liu^2,3, Zhaoyan Jiang, Aihua Gu²

¹State Key Laboratory of Reproductive Medicine, Institute of Toxicology,Nanjing Medical University, ²Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health,Nanjing Medical University, ³Center of Gallbladder Disease, Shanghai East Hospital, Institute of Gallstone Disease,Tongji University School of Medicine

Chapters

00:04Title
00:36Mitochondrial Fluorescence Intensity and Cellular ATP Detection
02:45Mitochondrial Membrane Potential Measurements
04:39Oxygen Consumption Rate (OCR) Measurements
06:22Results: Evaluation of Mitochondrial Function in Hepatocytes Exposed to β-HCH
07:43Conclusion

Summary

Automatic Translation

了解环境有机氯农药（OCPs）对肝细胞线粒体功能的影响，对于探讨OCP引起代谢紊乱的机理十分重要。本文提出了检测肝线粒体功能的详细方法。

Tags

Keywords: Mitochondrial Function Hepatocytes Organochlorine Pesticides ATP Level Oxygen Consumption HepG2 Cells Beta-hexachlorcyclohexane Mitochondrial Green Fluorescence Probe Confocal Microscopy Cellular ATP Luminometer Mitochondrial Membrane Potential JC1 Dye

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