/
/
Protocolo experimental para detectar la función mitocondrial en hepatocitos expuestos a plaguicidas organoclorados
A subscription to JoVE is required to view this content. Sign in or start your free trial.
1State Key Laboratory of Reproductive Medicine, Institute of Toxicology,Nanjing Medical University, 2Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health,Nanjing Medical University, 3Center of Gallbladder Disease, Shanghai East Hospital, Institute of Gallstone Disease,Tongji University School of Medicine
Chapters
- 00:04Title
- 00:36Mitochondrial Fluorescence Intensity and Cellular ATP Detection
- 02:45Mitochondrial Membrane Potential Measurements
- 04:39Oxygen Consumption Rate (OCR) Measurements
- 06:22Results: Evaluation of Mitochondrial Function in Hepatocytes Exposed to β-HCH
- 07:43Conclusion
Comprender la influencia de los pesticidas organoclorados ambientales (OCP) en la función mitocondrial en los hepatocitos es importante para explorar el mecanismo de los OCP que causan trastornos metabólicos. Este artículo presenta métodos detallados para detectar la función mitocondrial hepática.
