Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Guided Protocol for Fecal Microbial…
JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology
Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
 

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Article DOI: 10.3791/56845-v • 08:05 min • March 19th, 2018
March 19th, 2018 •
1, 1, 1, 1, 1, 1, 2, 1,3, 1,4
1Sheba Medical Center, 2Children's Hospital of Pittsburgh of UPMC, 3Ariel University, 4Cincinnati Children's Hospital Medical Center

Chapters

Summary
Automatic Translation

This manuscript describes a detailed standardized protocol of high-throughput 16S rRNA-amplicon sequencing. The protocol introduces an integrated, uniformed, feasible, and inexpensive protocol starting from fecal sample collection through data analyses. This protocol enables analysis of large numbers of samples with rigorous standards and several controls.

Tags

Keywords: 16S RRNA-Amplicon Sequencing Fecal Microbiome Microbial Composition Aerobic And Anaerobic Communities High-throughput Fecal Sample Processing DNA Extraction PCR Primer Design 96-well Plate Setup Triplicate Reactions PCR Master Mix
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter