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High-resolution Volume Imaging of Neurons by the Use of Fluorescence eXclusion Method and Dedicated Microfluidic Devices
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
High-resolution Volume Imaging of Neurons by the Use of Fluorescence eXclusion Method and Dedicated Microfluidic Devices

High-resolution Volume Imaging of Neurons by the Use of Fluorescence eXclusion Method and Dedicated Microfluidic Devices

DOI:
10.3791/56923-v

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09:11 min

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March 26, 2018

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, , , ,
1Laboratoire Physico-Chimie Curie, Institut Curie, Institut Pierre-Gilles de Gennes pour la microfluidique,Université PSL, CNRS, 2UMR 144 Institut Curie,Université PSL, CNRS

Chapters

  • 00:05Title
  • 00:47Fabrication of PDMS Chip
  • 01:51Fabrication of Patterned Coverslips
  • 03:13Chip Assembling and Final Implementation
  • 06:03Neuron Culture and Imaging
  • 07:14Results: Calibration of Fluorescence eXclusion Method Using a Photoresist Stripe as the Object of Interest and Neuronal Structures Volume Imaging
  • 08:17Conclusion

Summary

Automatic Translation

Automatic Translation

Volume is an important parameter regarding physiological and pathological characteristics of cells. We describe a fluorescent exclusion method allowing full-field measurement of in vitro neuronal volume with sub-micrometric axial resolution required for the analysis of neurites and dynamic structures implied in neuronal growth.

Tags

Keywords: Volume ImagingFluorescence ExclusionMicrofluidic DevicesNeuronNeuronal GrowthAxon DegenerationPDMSPhotoresistSpin-coatingMask AlignerPlasma Treatment

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