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A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells
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Cancer Research
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JoVE Journal Cancer Research
A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

DOI:
10.3791/56932-v

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11:15 min

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May 06, 2018

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, ,
1Neuro-oncology Research Group,VU Medical Center, 2Exosomes Research Group, Department of Pathology,VU Medical Center

Chapters

  • 00:04Title
  • 01:20Isolation of Tumor-secreted Extracellular Vesicles (EVs)
  • 04:00Generation of an Orthotopic Xenograft Mouse Model of Osteosarcoma
  • 06:44Assessment of Lung Nodule Number
  • 07:57Histological Analysis of GFP-positive MSCs in Mouse Tibias
  • 08:53Results: Analysis of Mice Injected with EV-educated MSCs
  • 10:31Conclusion

Summary

Automatic Translation

Automatic Translation

Direct injection of cancer-derived extracellular vesicles (EVs) leads to reprogramming of bone marrow supporting tumor progression; however, which cells mediate this effect is unclear. Herein, we describe a step-by-step protocol to investigate EV-mediated tumor-mesenchymal stem cell (MSC) interactions in vivo, revealing a crucial role for EV-educated MSCs in metastasis.

Tags

Preclinical Mouse ModelOsteosarcomaExtracellular Vesicle-mediated CommunicationTumorMesenchymal Stem CellsCancer BiologyEV IsolationDifferential CentrifugationUltracentrifugationCancer ProgressionMetastasisEV-depleted Medium143B Cells

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