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骨肉瘤前小鼠模型对肿瘤与间充质干细胞细胞外囊泡介导通信的定义

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Cancer Research

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JoVE Journal Cancer Research

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

Please note that all translations are automatically generated. Click here for the English version.

骨肉瘤前小鼠模型对肿瘤与间充质干细胞细胞外囊泡介导通信的定义

DOI:

10.3791/56932-v

•

11:15 min

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May 06, 2018

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Tonny Lagerweij*¹, Maria Pérez-Lanzón*², S. Rubina Baglio

¹Neuro-oncology Research Group,VU Medical Center, ²Exosomes Research Group, Department of Pathology,VU Medical Center

Chapters

00:04Title
01:20Isolation of Tumor-secreted Extracellular Vesicles (EVs)
04:00Generation of an Orthotopic Xenograft Mouse Model of Osteosarcoma
06:44Assessment of Lung Nodule Number
07:57Histological Analysis of GFP-positive MSCs in Mouse Tibias
08:53Results: Analysis of Mice Injected with EV-educated MSCs
10:31Conclusion

Summary

Automatic Translation

直接注射癌症衍生细胞外囊 (EVs) 导致骨髓支持肿瘤进展的重新编程;然而, 哪些细胞调解这种影响是不清楚的。在这里, 我们描述了一步一步的协议来调查 ev 介导的肿瘤间充质干细胞 (MSC) 相互作用的在体内, 揭示了一个关键的作用, 电动汽车教育的 MSCs 在转移。

Tags

Keywords: Preclinical Mouse Model Osteosarcoma Extracellular Vesicle-mediated Communication Tumor Mesenchymal Stem Cells Cancer Biology EV Isolation Differential Centrifugation Ultracentrifugation Cancer Progression Metastasis EV-depleted Medium 143B Cells

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