/

Developmental Biology

/

小鼠髓质前体 32-脑脊液-R 细胞的增殖和分化

JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Developmental Biology

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Please note that all translations are automatically generated. Click here for the English version.

小鼠髓质前体 32-脑脊液-R 细胞的增殖和分化

DOI:

10.3791/57033-v

•

10:21 min

•

February 21, 2018

•

Polina Zjablovskaja*^1,2, Petr Danek*¹, Miroslava Kardosova, Meritxell Alberich-Jorda²

¹Department of Hemato-Oncology,Institute of Molecular Genetics of the ASCR, ²Childhood Leukaemia Investigation Prague, Department of Paediatric Haematology and Oncology, 2nd Faculty of Medicine,Charles University

Chapters

00:04Title
00:58Media Preparation
01:24Production of Retrovirus
03:28Tritation of Virus Containing a Puromycin Reporter
05:04Transduction of 32D/G-CSF-R Cells
06:1032D/G-CSF-R Cell Differentiation Assay
07:32Results: Study of Proliferation and Differentiation of 32D/G-CSF-R Cells in Interleukin-3 and Granulocyte Colony Stimulating Factor Containing Media
09:21Conclusion

Summary

Automatic Translation

本文介绍了培养小鼠髓质前体32维/G-脑脊液 R 细胞系的详细规程, 并进行了病毒感染, 并进行了增殖和分化测定。该细胞系适合研究髓细胞发育, 以及兴趣基因在髓细胞生长和中性分化中的作用。

Tags

Keywords: Myeloid Precursor Cells 32D-G-CSF-R Cells Proliferation Differentiation Genetic Modification Retroviral Transduction Bosc23 Cells RPMI-1640 Medium Interleukin-3 MSCV Retroviral Construct PCL Echo Packaging Vector Polyethylenimine DMEM Medium Fetal Bovine Serum Virus Production Virus Titration

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

生产和使用慢病毒的有选择性地转导原少突胶质前体细胞<em>在体外</em>髓鞘测定

3D器官联合培养模型支持胸腺髓质上皮细胞增殖，分化和混杂基因表达

心脏分化的表观遗传调控胚胎干细胞和组织

制备及文化活动从成年和老年人类骨骼肌成肌前体细胞/成肌细胞主要的

在体外人骨髓间充质干细胞分化为心肌样细胞功能

人脑神经前体细胞神经发育表型的快速检测 (npc)

秀丽线虫生殖的计算分析研究细胞核、蛋白质和细胞骨架的分布

犬卵巢间充质干细胞的提取与分化

人类多能干细胞有效分化为肝细胞

人诱导多能干细胞体外生成体细胞衍生物的研究

Read Article