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胚荧光恢复后的染色质松动的光漂分析, 允许足月发展

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Developmental Biology

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JoVE Journal Developmental Biology

Zygotic Fluorescence Recovery After Photo-bleaching Analysis for Chromatin Looseness That Allows Full-term Development

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胚荧光恢复后的染色质松动的光漂分析, 允许足月发展

DOI:

10.3791/57068-v

•

10:30 min

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June 12, 2018

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Masatoshi Ooga³, Satoshi Funaya, Fugaku Aoki, Teruhiko Wakayama³

¹Faculty of Life and Environmental Sciences, Department of Biotechnology,University of Yamanashi, ²Department of Integrated Bioscience, Graduate School of Frontier Sciences,University of Tokyo, ³Advanced Biotechnology Center,University of Yamanashi

Chapters

00:05Title
01:01Preparation of Chamber and Microinjection Pipettes and Microinjection
03:42zFRAP Analysis
08:22Results: zFRAP Analysis of Chromatin Looseness for Full Term Development of Mice
10:00Conclusion

Summary

Automatic Translation

染色质松动似乎参与了卵裂球的发展潜力。然而, 目前尚不清楚染色质松动是否可以作为胚胎发育潜能的可靠指标。在这里, 我们描述了一个实验系统, 其中染色质松动评价受精卵可以发展到足月。

Tags

Zygotic FRAP ZFRAP Chromatin Looseness Embryonic Development EGFP-H2B MRNA Injection Microinjection Confocal Microscopy Photo-bleaching

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