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Chapters
- 00:05Title
- 01:01Setting Up Adult Zebrafish for Breeding
- 02:01Timed Matings – Collecting, Sorting, and Housing Zebrafish Embryos for Experiments
- 03:23Dechorionate, Deyolk, and Fix Zebrafish Embryos
- 06:09Staining DNA and FACS Sorting Embryos
- 08:00Results: Genome-wide DNA Replication Timing at High-resolution in Zebrafish
- 09:28Conclusion
Zebrafish were recently used as an in vivo model system to study DNA replication timing during development. Here is detailed the protocols for using zebrafish embryos to profile replication timing. This protocol can be easily adapted to study replication timing in mutants, individual cell types, disease models, and other species.
