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带谱系特异标记的间充质细胞脂肪分化电位的可视化与量化
JoVE Journal
Biology
Author Produced
This content is Free Access.
JoVE Journal Biology
Visualization and Quantification of Mesenchymal Cell Adipogenic Differentiation Potential with a Lineage Specific Marker
DOI:

13:26 min

March 31, 2018

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Chapters

  • 00:00Title
  • 01:55Inducing the differentiation of human adipose derived stromal cells
  • 03:36Staining for differentiation
  • 05:53Imaging cells using high content screening microscope
  • 08:23Image Analysis: Cell Scoring
  • 09:43Image Analysis: Transfluor
  • 10:43Representative results

Summary

Automatic Translation

传统的评价脂肪分化的方法既便宜又容易使用, 但并不特定于基因表达的变化。我们已经开发出一种用谱系特异标记将间充质细胞分化为成熟脂肪的实验。这种检测方法在基础研究和临床医学中有不同的应用。

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