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1School of Biological Sciences,The University of Auckland, 2Research School of Biology,The Australian National University, 3Auckland Cancer Society Research Centre, Faculty of Medical and Health Sciences,The University of Auckland, 4Department of Plastic Surgery,Counties Manukau District Health Board, 5Department of Surgery, Faculty of Medical and Health Sciences,The University of Auckland, 6Biomedical Imaging Research Unit, Faculty of Medical and Health Sciences,The University of Auckland, 7Maurice Wilkins Centre,The University of Auckland
Chapters
- 00:00Title
- 01:55Inducing the differentiation of human adipose derived stromal cells
- 03:36Staining for differentiation
- 05:53Imaging cells using high content screening microscope
- 08:23Image Analysis: Cell Scoring
- 09:43Image Analysis: Transfluor
- 10:43Representative results
Os métodos tradicionais de avaliação adipogenic diferenciação são baratos e fáceis de usar, mas não são específicos para alterações na expressão gênica. Nós desenvolvemos um ensaio para quantificar a diferenciação de células mesenquimais em adipócitos maduros, usando um marcador específico de linhagem. Este ensaio tem diversas aplicações através de pesquisa básica e clínica médica.
