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共聚焦显微镜通过图像相关谱揭示细胞表面受体聚集
JoVE Journal
Biology
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JoVE Journal Biology
Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy
DOI:

06:51 min

August 02, 2018

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Chapters

  • 00:04Title
  • 00:39Calculation of Image Correlation Spectroscopy
  • 03:43Batch Processing of ICS Datasets and Calculation of the Microscope s Beam Area
  • 04:59Results: Image Correlation Spectroscopy Detects Clusters on the Cell Surface of Adherent Cancer Cells
  • 05:55Conclusion

Summary

Automatic Translation

与细胞表面上的靶受体结合的抗体可以赋予构象和聚类改变。这些动态变化对表征靶细胞内药物的发展有影响。该协议利用共焦显微和图像相关光谱学通过 ImageJ/斐济, 以量化的程度, 受体聚类在细胞表面。

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