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小鼠单核细胞衍生树突状细胞的分离及与肿瘤免疫配合物的后体外活化

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Cancer Research

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JoVE Journal Cancer Research

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Please note that all translations are automatically generated. Click here for the English version.

小鼠单核细胞衍生树突状细胞的分离及与肿瘤免疫配合物的后体外活化

DOI:

10.3791/57188-v

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11:48 min

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May 31, 2018

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Nadine Santana-Magal*¹, Diana Rasoulouniriana*¹, Corey Saperia, Amit Gutwillig, Peleg Rider, Edgar G. Engleman, Yaron Carmi

¹Department of Pathology, Sackler School of Medicine,Tel-Aviv University, ²Department of Pathology, School of Medicine,Stanford University

Chapters

00:04Title
01:11Isolation of Tumor Associated Monocyte-derived DC
04:07Isolation of Monocyte-derived DC from Peripheral Blood
06:54Preparation of Tumor-IgG Immune Complexes Followed by Activation of MoDC with Tumor-IgG
08:58Results: Isolation and Analysis of the Activation of MoDC with the Tumor Immune Complex
11:02Conclusion

Summary

Automatic Translation

单核细胞衍生的 DC (MoDC) 可以感觉到少量的危险相关分子, 因此很容易被引。我们提供了一个详细的协议, 以隔离 MoDC 从血液和肿瘤和他们的活化与免疫复合体, 同时强调应考虑的关键预防措施, 以避免其过早激活。

Tags

Keywords: Mouse Monocyte-derived Dendritic Cells Tumor Isolation Activation Tumor Immune Complexes Myeloid Cells Cell Culture Magnetic Beads Centrifugation Density Gradient Medium

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